Sie sind hier: Startseite Microscopy

Microscopy and Image Analysis - Overview

Below you can find an overview of systems located in the ZTZ building in the Lighthouse main lab, as well as in the BiMiC of the IMITATE building. Detailed information about individual system configurations can also be found in the Lighthouse calendar.

Complex Microscope Systems

Slide Scanning

Long-Term Live Cell Imaging

Additional Widefield Systems

Image Analysis

Biosafety Information

 

Complex Microscope Systems

akoya_vp

Spatial Biology / Highly Multiplexed Microscopy

Lighthouse has recently installed an

Akoya Phenocycler-Fusion (CODEX) (ZTZ)

which allows the analysis of up to 100+ markers on a single slide.

More information about the Phenocycler-Fusion

 

airysectionConfocal and Lightsheet Microscopy

ZTZ: Zeiss LSM 880 with AiryScan

ZTZ: Zeiss LSM 710

IMTATE: Zeiss LSM 980 NLO (multiphoton) with AiryScan

IMTATE: Zeiss CellDiscoverer 7 with LSM 900 AiryScan Scanhead

IMTATE: Zeiss Lightsheet 7

More information about the microscopes

 

 

High Content Screening (HCS)/ Automated Microscopy

Microscopes for Image Cytometry/Automated Microscopy/HCS:

ZTZ: Olympus scanR High Content Screening Station

IMITATE: Zeiss CellDiscoverer 7 with LSM 900 AiryScan

More information about the HCS systems

 

 

Slide Scanning

 

axioscan

Zeiss AxioScan 7 (IMITATE)

High capacity slide scanner for fluorescence or brightfield (IHC) samples. (Located in the IMITATE building)

 Configuration

 

 

 

Long-Term Live Cell Imaging

incucyte

Lighthouse has a Sartorius Incucyte S3 system, which is an automated microscope contained within an incubator. It allows the independent imaging and analysis of up to 6 plates or experiments simultaneously.

More information about the Incucyte system.

 

 

 

Additional Widefield Systems

There are also several other microscope systems available for you to use:

 

axioimager

Zeiss AxioImager M2m (ZTZ)

Upright widefield microscope for standard immunohistochemistry and fluorescence microscopy.

 Configuration

 

 

axioobserver1Zeiss AxioObserver 1 (ZTZ)

Zeiss AxioOvserver 2 (ZTZ)

Inverted fluorescence microscopes with on-stage climate chamber or heated stage, and motorized XYZ stages. Tiling, Z-stacks, and more.

 

 stereo

Zeiss Discovery.V8 (ZTZ)

Stereomicroscope. Brightfield and fluorescence (GFP/RFP).

 

 

Image Analysis

lambda4Several workstations in the facility are reserved for offline analysis. Available software includes:

  • Imaris - 3D reconstruction and analysis from Oxford Instruments
  • AutoQuant / AutoDeblur - Deconvolution and 3D reconstruction
  • Metamorph - Image Analysis
  • Zeiss Zen Blue / Black
  • Olympus scanR analysis, with Deep Learning AI
  • Cell Profiler
  • ImageJ / FiJi
  • Akoya Inform Software
  • QuPath
  • CytoMap

 

Biosafety Information

Live cell imaging of samples of biosafety level S2 (Gentechnikgesetz/ Infektionsschutzgesetz) requires that you first fill out a

Lighthouse Biosafety Form

Note:
It is not necessary to fill out the checklist for every appointment. However, it should be filled out for each project, including experiments involving different cell types, treatments or sample origin.

 

 

Additional Links

General Microscopy and Image Analysis Links

Lighthouse Microscopy Calendar

Home