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Quantitative Real-Time PCR

References

MIQE Guidelines and Digital MIQE Guidelines - These are where to start when first planning your QPCR and dPCR experiments (Minimum Information for Publication of Quantitative Real-Time PCR Experiments), respectively.

Gene Quantification Home Page - this site has developed into a very useful QPCR reference web site. It summarizes many of the technical aspects of real-time PCR and has numerous software and reference downloads useful for newcomers and experts alike.

Normfinder is an algorithm for identifying optimal normalization genes  from among a set of candidates. It ranks the set of candidate normalization genes according to their expression stability in a given sample set and given experimental design.

geNorm is a very useful collection of VBA macros for Microsoft Excel which can help you determine the most stable reference genes for your experiments as well as help with normalization. 

The Ensembl Genome Browser is the home to various genome databases and is a very good reference when searching for splice site locations, etc.

Digital PCR

Stilla's Gene Pi Digital PCR Learning Center is an informative website which you can use to learn more about digital PCR, including a number of statistical tools, such as how to determine the limit of blank (LOB) and the limit of detection (LOD) of your assay, multiple tutorials, and other useful information.

The Gene Quantification Homepage also has an informative section on digital PCR.

YouTube video explaining droplet digital PCR.

Primer Design

Beacon Designer and its online tutorial - to help you with primer/probe design for both QPCR/dPCR reactions, Lighthouse has a license available for use in the main lab.

In addition to Beacon Designer, there are several online sites for designing primer/probe sets for QPCR/dPCR:

Primer 3 - online primer and probe design site from MIT's Whitehead Institute.

PrimerBank  - another public databank for PCR primers designed for gene expression quantification.

Company Sites

LightCycler 480 from Roche Applied Science.

Stilla Naica Crystal Digital PCR system from Stilla Technologies.

QIAcuity Digital PCR System from Qiagen.

Actome website- including more information about their Protein Interaction Coupling (PICO) technology