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Flow Cytometry and Cell Sorting


Flow Cytometry - A Basic Introduction - an online version of Michael Ormerod's book, hosted by Denovo Software. If you are new to flow cytometry, welcome. This is a good place to start.

Practical Flow Cytometry - an online version of Howard Shapiro's classic book, hosted by BeckmanCoulter. Considered by many to be the "Bible of Flow Cytometry".

Not just for immunologists: Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), from Andrea Cossarizza et al. The guidelines cover theory and practical tips for flow cytometry applications, as well as important aspects of data analysis. Also contains useful tables on human and murine cell phenotypes.

Purdue University Cytometry Labs - Informative site including general cytometry links as well as the very helpful  Cytometry e-mail list archives.

Checkout Mario Roederer's tutorial on correct compensation, Compensation: yet another perspective, and useful protocol collection on conjugation of monoclonal antibodies.

The Flow Cytometry Core of the Blizard Institute of Cell and Molecular Science has an excellent collection of applications and protocols for flow cytometry.

Spectral Databases

Some useful sites containing fluorophore excitation/emission spectra databases are

Spectra Viewer from Fluorofinder is useful in that it is manufacturer-independent

Fluorescence Spectrum Viewer from Becton Dickinson.

Fluorescence Spectraviewer from ThermoFisher/eBioscience (formerly Molecular Probes).

Spectraviewer from the folks at the Imaging Facility of the MPI for Brain Research in Frankfurt.

Fluorescent Protein Resource Center - in addition to excitation/emission maxima contains other useful information about fluorescent proteins and their properties. - comprehensive database with useful spectral library. 

Cytometry Societies

ISAC - Home page of the International Society for the Advancement of Cytometry.

DGfZ - Home page of the German Society for Cytometry. - Network of cytometrists from across Germany. 

Software and Online Tools

Fluorofinder - here you can find configurations for all Lighthouse analysers and cell sorters, as well as very useful help with panel design and many other tools.

FlowJo - You can access this flow cytometry analysis software available on the Lighthouse FlowJo analysis computers. The official FlowJo website has several online tutorials. You can also download a demo version directly from the website  here.

FCS Express - The FCS Express software has been installed on the Lighthouse FlowJo2 analysis computer. Tutorials are available on the website, along with a demo version for download.

CytoSpec Software - a free flow cytometry data analysis software from Purdue University Cytometry Laboratories. It is an easy to use program for hyperspectral analysis, and it can do PCA on standard listmode files as well.

Flowing Software - a flow cytometry data analysis softare from the Turku Centre for Biotechnology which is free for academic users. Tutorials and documentation are available online.

Red Matter App - Free flow cytometry software which can be used on any device.

Antibody Directories

Linscott's Directory - Directory of commercially available antibodies.

Antikoerper Online - Directory of commercially available antibodies.

Company Sites

BD Biosciences - Where you can get more information about the Aria cell sorters and the LSR Fortessa analysers.

Beckman Coulter  - Where you can get more information about the MoFlo Astrios cell sorter and the Gallios/Navios analysers.

Sony Biotechnology - Where you can get more information about the ID7000C and SP6800ZE Spectral Analysers.

Cytek - For more information about the Cytek Aurora spectral analyzer.