Long-Term Live Cell Imaging
Sartorius Incucyte S3
The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development. Available objectives include 4x, 10x and 20x. These objectives are compatible with most standard cell culture plate formats and plates of to 96- or 384-wells are also supported.
List of supported plates/substrates
Channels available for imaging and analysis are standard brightfield/phase contrast, as well as two channels for fluorescence.
Details about fluorescence wavelengths can be seen below:
| Color | Excitation | Emission | Possible Fluorochromes |
| Green Fluorescence | 440-480 nm | 504-544 nm | GFP, YFP, CellTracker Green, CFSE, FITC |
| Red Fluorescence | 565-605 nm | 625-705 nm | mRFP, mKate2, mCherry, CellTracker Red, AlexaFluor 594 |
Note: The red fluroescence channel is suboptimal for the detection of fluorescence proteins such as td tomato and turbo RFP.
Further Information
General Microscopy Links of Interest